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full length human aid  (Addgene inc)


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    Addgene inc full length human aid
    Full Length Human Aid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+human+aid/pmc04006916-216-29-36?v=Addgene+inc
    Average 90 stars, based on 2 article reviews
    full length human aid - by Bioz Stars, 2026-07
    90/100 stars

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    <t>AID</t> induces SHM in CHO cells. (A) Murine Vn/ECMV γ2a construct transfected into CHO cells to study SHM. As described in ref. 17, this heavy chain Ig construct has replaced the intronic μ enhancer with a CMV enhancer and contains a TAG nonsense codon within an RGYW hotspot motif at codon 38. (B Left) CHO clone CHO-LC18 (see Materials and Methods) stably transfected with heavy and light chain Ig genes was transfected with empty vector (○) or the <t>hAID</t> construct (•). Depending on expression of AID (see below), data were distributed into AID-negative (AID−) and AID-positive (AID+) columns. Frequency of nonsense revertants, as detected with the ELISA spot assay, is plotted. (Right) Ten subclones of CHO clones A.3 and A.9 were further analyzed by using the ELISA spot assay to calculate mutation rates by fluctuation analysis. (C) Northern blots for hAID and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in CHO transfectants. Clone numbers correspond to numbers in B Left.
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    AID induces SHM in CHO cells. (A) Murine Vn/ECMV γ2a construct transfected into CHO cells to study SHM. As described in ref. 17, this heavy chain Ig construct has replaced the intronic μ enhancer with a CMV enhancer and contains a TAG nonsense codon within an RGYW hotspot motif at codon 38. (B Left) CHO clone CHO-LC18 (see Materials and Methods) stably transfected with heavy and light chain Ig genes was transfected with empty vector (○) or the hAID construct (•). Depending on expression of AID (see below), data were distributed into AID-negative (AID−) and AID-positive (AID+) columns. Frequency of nonsense revertants, as detected with the ELISA spot assay, is plotted. (Right) Ten subclones of CHO clones A.3 and A.9 were further analyzed by using the ELISA spot assay to calculate mutation rates by fluctuation analysis. (C) Northern blots for hAID and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in CHO transfectants. Clone numbers correspond to numbers in B Left.

    Journal:

    Article Title: Somatic hypermutation of the AID transgene in B and non-B cells

    doi: 10.1073/pnas.192442899

    Figure Lengend Snippet: AID induces SHM in CHO cells. (A) Murine Vn/ECMV γ2a construct transfected into CHO cells to study SHM. As described in ref. 17, this heavy chain Ig construct has replaced the intronic μ enhancer with a CMV enhancer and contains a TAG nonsense codon within an RGYW hotspot motif at codon 38. (B Left) CHO clone CHO-LC18 (see Materials and Methods) stably transfected with heavy and light chain Ig genes was transfected with empty vector (○) or the hAID construct (•). Depending on expression of AID (see below), data were distributed into AID-negative (AID−) and AID-positive (AID+) columns. Frequency of nonsense revertants, as detected with the ELISA spot assay, is plotted. (Right) Ten subclones of CHO clones A.3 and A.9 were further analyzed by using the ELISA spot assay to calculate mutation rates by fluctuation analysis. (C) Northern blots for hAID and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in CHO transfectants. Clone numbers correspond to numbers in B Left.

    Article Snippet: Full-length human AID (hAID) cloned into the pCEP4 vector (Invitrogen) has been described ( 8 ).

    Techniques: Construct, Transfection, Stable Transfection, Plasmid Preparation, Expressing, Enzyme-linked Immunosorbent Assay, Spot Test, Clone Assay, Mutagenesis, Northern Blot